Functional Characterization of Cells Separated From Suspensions of Hodgkin Disease Tumor Cells in an Isokinetic Gradient
نویسندگان
چکیده
Suspensions of cells from Hodgkin disease which formed nonimmune E rosettes with tumors were separated by velocity sedisheep red blood cells, they showed a lack mentation in an isokinetic Ficoll gradient. of responsiveness to phytohemagglutinin The separated cells were cultured with which was almost complete in all but one mitogens. The modal population of lympatient. Lymphocytes which had been obphocytes from the tumor contained 50%tamed in suspension and purified by iden75% of all lymphocytes separated from tical methods from tonsils and spleens the tumor of each studied patient. Dcwithout tumors did respond to stimulation spite the fact that these cells appeared to by phytohemagglutinin. be mature lymphocytes, the majority of
منابع مشابه
Functional characterization of cells separated from suspensions of Hodgkin diseases tumor cells in an isokinetic gradient.
Suspensions of cells from Hodgkin disease tumors were separated by velocity sedimentation in an isokinetic Ficall gradient. The separated cells were cultured with mitogens. The modal population of lymphocytes from the tumor contained 50%-75% of all lymphocytes separated from the tumor of each studied patient. Despite the fact that these cells appeared to be mature lymphocytes, the majority of ...
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